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| Presentation | 2019-01-22 10:30 [Poster Presentation] The mechanism of denaturation and amplification of DNA by audible frequency vibration Ryu Kobayashi, Keitaro Shimizu, Tadzunu Suzuki, Seiji Yoneda, Shigeo Yamaguchi (kanagawa Univ.)  US2018-80 |
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| Abstract | (in Japanese) | (See Japanese page) |
| (in English) | In the PCR method, a strong DNA amplification technology, DNA is denatured from double strand to single strand at high temperature. However, during the process of PCR, DNA and enzymes are damaged by the condition of high temperature of 94℃. We have so far proposed and developed a new DNA amplification method using vibration instead of using high temperature state. In our method, DNA is denatured by vibrating the DNA solution at audible frequencies using a wave transducer. To analyze the results of DNA amplification by vibration, a microchip-type electrophoresis was used. Furthermore, the energy applied to DNA by vibration was estimated, and the relationship between the estimated values and the amplification of DNA was discussed. | |
| Keyword | (in Japanese) | (See Japanese page) |
| (in English) | DNA / PCR / Vibration / Denaturation / / / / | |
| Reference Info. | IEICE Tech. Rep., vol. 118, no. 409, US2018-80, pp. 9-12, Jan. 2019. | |
| Paper # | US2018-80 | |
| Date of Issue | 2019-01-15 (US) | |
| ISSN | Print edition: ISSN 0913-5685 Online edition: ISSN 2432-6380 | |
| Copyright and reproduction |
All rights are reserved and no part of this publication may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopy, recording, or any information storage and retrieval system, without permission in writing from the publisher. Notwithstanding, instructors are permitted to photocopy isolated articles for noncommercial classroom use without fee. (License No.: 10GA0019/12GB0052/13GB0056/17GB0034/18GB0034) | |
| Download PDF | US2018-80 |
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| Conference Information | |
| Committee | EA US |
| Conference Date | 2019-01-22 - 2019-01-23 |
| Place (in Japanese) | (See Japanese page) |
| Place (in English) | Doshisha Univ. |
| Topics (in Japanese) | (See Japanese page) |
| Topics (in English) | [Joint Meeting on Acoustics and Ultrasonics Subsociety] Engineering/Electro Acoustics, Ultrasonics, and Related Topics |
| Paper Information | |
| Registration To | US |
| Conference Code | 2019-01-EA-US |
| Language | Japanese |
| Title (in Japanese) | (See Japanese page) |
| Sub Title (in Japanese) | (See Japanese page) |
| Title (in English) | The mechanism of denaturation and amplification of DNA by audible frequency vibration |
| Sub Title (in English) | |
| Keyword(1) | DNA |
| Keyword(2) | PCR |
| Keyword(3) | Vibration |
| Keyword(4) | Denaturation |
| Keyword(5) | |
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| Keyword(8) | |
| 1st Author's Name | Ryu Kobayashi |
| 1st Author's Affiliation | Kanagawa University (kanagawa Univ.) |
| 2nd Author's Name | Keitaro Shimizu |
| 2nd Author's Affiliation | Kanagawa University (kanagawa Univ.) |
| 3rd Author's Name | Tadzunu Suzuki |
| 3rd Author's Affiliation | Kanagawa University (kanagawa Univ.) |
| 4th Author's Name | Seiji Yoneda |
| 4th Author's Affiliation | Kanagawa University (kanagawa Univ.) |
| 5th Author's Name | Shigeo Yamaguchi |
| 5th Author's Affiliation | Kanagawa University (kanagawa Univ.) |
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| Speaker | Author-1 |
| Date Time | 2019-01-22 10:30:00 |
| Presentation Time | 120 minutes |
| Registration for | US |
| Paper # | US2018-80 |
| Volume (vol) | vol.118 |
| Number (no) | no.409 |
| Page | pp.9-12 |
| #Pages | 4 |
| Date of Issue | 2019-01-15 (US) |
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