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| Presentation | 2017-06-16 10:30 Basic study on usefulness of electrically induced microbubbles for in vitro sonoporation Akira Tokoyo, Nobuki Kudo (Hokkaido Univ.)  MBE2017-11 |
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| Abstract | (in Japanese) | (See Japanese page) |
| (in English) | We have been studying a sonoporation technique that uses pulsed ultrasound and microbubbles for gene or drug transduction into cells. In vitro sonoporation experiments, monolayer cells cultured on a coverslip were dipped into microbubbles suspension to attach the bubbles on the cells, and then the cells were irradiated by ultrasound to induce cell membrane perforation. Microbubbles of an ultrasound contrast agent are widely used for sonoporation studies, but it is difficult to control sizes and locations of bubbles that attaches to cells. In this study, we investigated a technique to generate microbubbles with a small variation in diameter and with several types of shell material by electrically induced. In vitro sonoporation experiment was carried out, indicating that the bubbles can induce sonoporation effect of cultured cells. | |
| Keyword | (in Japanese) | (See Japanese page) |
| (in English) | sonoporation / ultrasound / microbubble / electrically induced method / cell engineering / / / | |
| Reference Info. | IEICE Tech. Rep., vol. 117, no. 94, MBE2017-11, pp. 1-6, June 2017. | |
| Paper # | MBE2017-11 | |
| Date of Issue | 2017-06-09 (MBE) | |
| ISSN | Print edition: ISSN 0913-5685 Online edition: ISSN 2432-6380 | |
| Copyright and reproduction |
All rights are reserved and no part of this publication may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopy, recording, or any information storage and retrieval system, without permission in writing from the publisher. Notwithstanding, instructors are permitted to photocopy isolated articles for noncommercial classroom use without fee. (License No.: 10GA0019/12GB0052/13GB0056/17GB0034/18GB0034) | |
| Download PDF | MBE2017-11 |
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| Conference Information | |
| Committee | MBE |
| Conference Date | 2017-06-16 - 2017-06-16 |
| Place (in Japanese) | (See Japanese page) |
| Place (in English) | Hokkaido University |
| Topics (in Japanese) | (See Japanese page) |
| Topics (in English) | ME, general |
| Paper Information | |
| Registration To | MBE |
| Conference Code | 2017-06-MBE |
| Language | Japanese |
| Title (in Japanese) | (See Japanese page) |
| Sub Title (in Japanese) | (See Japanese page) |
| Title (in English) | Basic study on usefulness of electrically induced microbubbles for in vitro sonoporation |
| Sub Title (in English) | |
| Keyword(1) | sonoporation |
| Keyword(2) | ultrasound |
| Keyword(3) | microbubble |
| Keyword(4) | electrically induced method |
| Keyword(5) | cell engineering |
| Keyword(6) | |
| Keyword(7) | |
| Keyword(8) | |
| 1st Author's Name | Akira Tokoyo |
| 1st Author's Affiliation | Hokkaido University (Hokkaido Univ.) |
| 2nd Author's Name | Nobuki Kudo |
| 2nd Author's Affiliation | Hokkaido University (Hokkaido Univ.) |
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| Speaker | Author-1 |
| Date Time | 2017-06-16 10:30:00 |
| Presentation Time | 25 minutes |
| Registration for | MBE |
| Paper # | MBE2017-11 |
| Volume (vol) | vol.117 |
| Number (no) | no.94 |
| Page | pp.1-6 |
| #Pages | 6 |
| Date of Issue | 2017-06-09 (MBE) |
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