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| Presentation | 2017-03-14 14:00 Estimation of refractive index in the living brain by optimizing spherical aberration. Hiromu Monai (RIKEN), Yoshihiro Ue, Kaori Higuchi, Daisuke Nishiwaki, Tetsuya Tajima, Kenya Okazaki (RIKEN/OLYMPUS), Hiroshi Hama, Hirase Hajime, Atsushi Miyawaki (RIKEN)  NC2016-93 |
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| Abstract | (in Japanese) | (See Japanese page) |
| (in English) | The high-resolution imaging of fine structures such as neurites requires objectives with high numerical apertures (NAs). However, spherical aberration (SA) due to the mismatch of refractive indices between an immersion medium and samples, often distorts acquired images. Whereas a correction collar attached to high NAs objectives has been designed to compensate SA, its manual adjustment and acquisition of the best image of a given samples is the most difficult mission. To solve the problem, we have developed SA compensation system, by motorizing collection collar and formulating an algorithm which calculates the optimum collection collar position based on contrasts of images. This system allows quantitative analysis of fine structures of cerebral cortex in vivo. Furthermore, as this system enables us to estimate the refractive index of a given samples on the basis of the compensation degree, we found the cortical structure could be optically heterogeneous along the z-axis in the living brain. | |
| Keyword | (in Japanese) | (See Japanese page) |
| (in English) | correction collar / refractive index (RI) / in vivo imaging / two-photon microscopy / / / / | |
| Reference Info. | IEICE Tech. Rep., vol. 116, no. 521, NC2016-93, pp. 171-176, March 2017. | |
| Paper # | NC2016-93 | |
| Date of Issue | 2017-03-06 (NC) | |
| ISSN | Print edition: ISSN 0913-5685 Online edition: ISSN 2432-6380 | |
| Copyright and reproduction |
All rights are reserved and no part of this publication may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopy, recording, or any information storage and retrieval system, without permission in writing from the publisher. Notwithstanding, instructors are permitted to photocopy isolated articles for noncommercial classroom use without fee. (License No.: 10GA0019/12GB0052/13GB0056/17GB0034/18GB0034) | |
| Download PDF | NC2016-93 |
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| Conference Information | |
| Committee | MBE NC |
| Conference Date | 2017-03-13 - 2017-03-14 |
| Place (in Japanese) | (See Japanese page) |
| Place (in English) | Kikai-Shinko-Kaikan Bldg. |
| Topics (in Japanese) | (See Japanese page) |
| Topics (in English) | |
| Paper Information | |
| Registration To | NC |
| Conference Code | 2017-03-MBE-NC |
| Language | Japanese |
| Title (in Japanese) | (See Japanese page) |
| Sub Title (in Japanese) | (See Japanese page) |
| Title (in English) | Estimation of refractive index in the living brain by optimizing spherical aberration. |
| Sub Title (in English) | |
| Keyword(1) | correction collar |
| Keyword(2) | refractive index (RI) |
| Keyword(3) | in vivo imaging |
| Keyword(4) | two-photon microscopy |
| Keyword(5) | |
| Keyword(6) | |
| Keyword(7) | |
| Keyword(8) | |
| 1st Author's Name | Hiromu Monai |
| 1st Author's Affiliation | RIKEN (RIKEN) |
| 2nd Author's Name | Yoshihiro Ue |
| 2nd Author's Affiliation | .RIKEN/OLYMPUS Corp (RIKEN/OLYMPUS) |
| 3rd Author's Name | Kaori Higuchi |
| 3rd Author's Affiliation | .RIKEN/OLYMPUS Corp (RIKEN/OLYMPUS) |
| 4th Author's Name | Daisuke Nishiwaki |
| 4th Author's Affiliation | .RIKEN/OLYMPUS Corp (RIKEN/OLYMPUS) |
| 5th Author's Name | Tetsuya Tajima |
| 5th Author's Affiliation | .RIKEN/OLYMPUS Corp (RIKEN/OLYMPUS) |
| 6th Author's Name | Kenya Okazaki |
| 6th Author's Affiliation | .RIKEN/OLYMPUS Corp (RIKEN/OLYMPUS) |
| 7th Author's Name | Hiroshi Hama |
| 7th Author's Affiliation | RIKEN (RIKEN) |
| 8th Author's Name | Hirase Hajime |
| 8th Author's Affiliation | RIKEN (RIKEN) |
| 9th Author's Name | Atsushi Miyawaki |
| 9th Author's Affiliation | RIKEN (RIKEN) |
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| Speaker | Author-1 |
| Date Time | 2017-03-14 14:00:00 |
| Presentation Time | 25 minutes |
| Registration for | NC |
| Paper # | NC2016-93 |
| Volume (vol) | vol.116 |
| Number (no) | no.521 |
| Page | pp.171-176 |
| #Pages | 6 |
| Date of Issue | 2017-03-06 (NC) |
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