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Paper Abstract and Keywords
Presentation 2017-03-14 14:00
Estimation of refractive index in the living brain by optimizing spherical aberration.
Hiromu Monai (RIKEN), Yoshihiro Ue, Kaori Higuchi, Daisuke Nishiwaki, Tetsuya Tajima, Kenya Okazaki (RIKEN/OLYMPUS), Hiroshi Hama, Hirase Hajime, Atsushi Miyawaki (RIKEN) NC2016-93
Abstract (in Japanese) (See Japanese page) 
(in English) The high-resolution imaging of fine structures such as neurites requires objectives with high numerical apertures (NAs). However, spherical aberration (SA) due to the mismatch of refractive indices between an immersion medium and samples, often distorts acquired images. Whereas a correction collar attached to high NAs objectives has been designed to compensate SA, its manual adjustment and acquisition of the best image of a given samples is the most difficult mission. To solve the problem, we have developed SA compensation system, by motorizing collection collar and formulating an algorithm which calculates the optimum collection collar position based on contrasts of images. This system allows quantitative analysis of fine structures of cerebral cortex in vivo. Furthermore, as this system enables us to estimate the refractive index of a given samples on the basis of the compensation degree, we found the cortical structure could be optically heterogeneous along the z-axis in the living brain.
Keyword (in Japanese) (See Japanese page) 
(in English) correction collar / refractive index (RI) / in vivo imaging / two-photon microscopy / / / /  
Reference Info. IEICE Tech. Rep., vol. 116, no. 521, NC2016-93, pp. 171-176, March 2017.
Paper # NC2016-93 
Date of Issue 2017-03-06 (NC) 
ISSN Print edition: ISSN 0913-5685  Online edition: ISSN 2432-6380
All rights are reserved and no part of this publication may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopy, recording, or any information storage and retrieval system, without permission in writing from the publisher. Notwithstanding, instructors are permitted to photocopy isolated articles for noncommercial classroom use without fee. (License No.: 10GA0019/12GB0052/13GB0056/17GB0034/18GB0034)
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Conference Information
Committee MBE NC  
Conference Date 2017-03-13 - 2017-03-14 
Place (in Japanese) (See Japanese page) 
Place (in English) Kikai-Shinko-Kaikan Bldg. 
Topics (in Japanese) (See Japanese page) 
Topics (in English)  
Paper Information
Registration To NC 
Conference Code 2017-03-MBE-NC 
Language Japanese 
Title (in Japanese) (See Japanese page) 
Sub Title (in Japanese) (See Japanese page) 
Title (in English) Estimation of refractive index in the living brain by optimizing spherical aberration. 
Sub Title (in English)  
Keyword(1) correction collar  
Keyword(2) refractive index (RI)  
Keyword(3) in vivo imaging  
Keyword(4) two-photon microscopy  
1st Author's Name Hiromu Monai  
1st Author's Affiliation RIKEN (RIKEN)
2nd Author's Name Yoshihiro Ue  
2nd Author's Affiliation .RIKEN/OLYMPUS Corp (RIKEN/OLYMPUS)
3rd Author's Name Kaori Higuchi  
3rd Author's Affiliation .RIKEN/OLYMPUS Corp (RIKEN/OLYMPUS)
4th Author's Name Daisuke Nishiwaki  
4th Author's Affiliation .RIKEN/OLYMPUS Corp (RIKEN/OLYMPUS)
5th Author's Name Tetsuya Tajima  
5th Author's Affiliation .RIKEN/OLYMPUS Corp (RIKEN/OLYMPUS)
6th Author's Name Kenya Okazaki  
6th Author's Affiliation .RIKEN/OLYMPUS Corp (RIKEN/OLYMPUS)
7th Author's Name Hiroshi Hama  
7th Author's Affiliation RIKEN (RIKEN)
8th Author's Name Hirase Hajime  
8th Author's Affiliation RIKEN (RIKEN)
9th Author's Name Atsushi Miyawaki  
9th Author's Affiliation RIKEN (RIKEN)
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Date Time 2017-03-14 14:00:00 
Presentation Time 25 
Registration for NC 
Paper # IEICE-NC2016-93 
Volume (vol) IEICE-116 
Number (no) no.521 
Page pp.171-176 
#Pages IEICE-6 
Date of Issue IEICE-NC-2017-03-06 

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