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Paper Abstract and Keywords
Presentation 2016-03-22 11:25
Image classification of astrocytes for pre- and post-hypoxia adaptation using deep convolutional neural network
Sosuke Tanaka, Masahiro Nitta, Kazuto Masamoto, Yoichi Miyawaki (UEC Tokyo) NC2015-91
Abstract (in Japanese) (See Japanese page) 
(in English) Abstract Astrocytes are a type of glial cells that regulate transportation of nutrition from blood to neurons. Previous studies showed astrocytes change their shape in case of pathological conditions such as hypoxia, suggesting functional roles in microvasculature remodeling in response to environmental changes in the brain. However, it remains unclear what morphological features are specifically influenced by such pathological conditions. In this study, we focused on examples of hypoxia-adapting astrocytes and proposed a novel approach using DCNN (Deep Convolutional Neural Network) to extract morphological features of the astrocytes that change between pre- and post-hypoxia adaptation. The image data of astrocyte, measured by the two-photon microscopy, was analyzed by DCNN and image features represented in a higher layer was used to predict whether each of given astrocyte images corresponds to pre- or post-hypoxia adaptation. Results showed that the prediction performance was accurate (> 95%) for DCNN-extracted features, significantly higher than for other simple image features. Analyses of extracted features further showed that only a small number of image features were important for the prediction. These results suggest that DCNN-extracted image features contain useful information to identify morphological changes of astrocytes during hypoxic adaptation
Keyword (in Japanese) (See Japanese page) 
(in English) Astrocyte / Deep convolutional neural network / Hypoxia / Image feature / Cellular morphology / Two-photon microscopy / Support vector machine / Machine learning  
Reference Info. IEICE Tech. Rep., vol. 115, no. 514, NC2015-91, pp. 125-130, March 2016.
Paper # NC2015-91 
Date of Issue 2016-03-15 (NC) 
ISSN Print edition: ISSN 0913-5685    Online edition: ISSN 2432-6380
Copyright
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All rights are reserved and no part of this publication may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopy, recording, or any information storage and retrieval system, without permission in writing from the publisher. Notwithstanding, instructors are permitted to photocopy isolated articles for noncommercial classroom use without fee. (License No.: 10GA0019/12GB0052/13GB0056/17GB0034/18GB0034)
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Conference Information
Committee MBE NC  
Conference Date 2016-03-22 - 2016-03-23 
Place (in Japanese) (See Japanese page) 
Place (in English) Tamagawa University 
Topics (in Japanese) (See Japanese page) 
Topics (in English)  
Paper Information
Registration To NC 
Conference Code 2016-03-MBE-NC 
Language Japanese 
Title (in Japanese) (See Japanese page) 
Sub Title (in Japanese) (See Japanese page) 
Title (in English) Image classification of astrocytes for pre- and post-hypoxia adaptation using deep convolutional neural network 
Sub Title (in English)  
Keyword(1) Astrocyte  
Keyword(2) Deep convolutional neural network  
Keyword(3) Hypoxia  
Keyword(4) Image feature  
Keyword(5) Cellular morphology  
Keyword(6) Two-photon microscopy  
Keyword(7) Support vector machine  
Keyword(8) Machine learning  
1st Author's Name Sosuke Tanaka  
1st Author's Affiliation The University of Electro-Communications (UEC Tokyo)
2nd Author's Name Masahiro Nitta  
2nd Author's Affiliation The University of Electro-Communications (UEC Tokyo)
3rd Author's Name Kazuto Masamoto  
3rd Author's Affiliation The University of Electro-Communications (UEC Tokyo)
4th Author's Name Yoichi Miyawaki  
4th Author's Affiliation The University of Electro-Communications (UEC Tokyo)
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Speaker Author-1 
Date Time 2016-03-22 11:25:00 
Presentation Time 25 minutes 
Registration for NC 
Paper # NC2015-91 
Volume (vol) vol.115 
Number (no) no.514 
Page pp.125-130 
#Pages
Date of Issue 2016-03-15 (NC) 


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