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| Presentation | 2014-11-22 09:50 Simple and Rapid Renal Function Analysis Using Newly Conducted Urea Microassay Toru Yokoyama (Sapporo College of Medical, Welfare and Dental Tech.)  MBE2014-69 |
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| Abstract | (in Japanese) | (See Japanese page) |
| (in English) | The author describes a newly conducted quantitative and sensitive microassay for urea determination using an urease-indophenol method. This assay uses the catalyzed indophenol reaction for the determination of ammonia. The procedure was adapted for the determination of urea after hydrolysis with urease. The final product in this reaction, which is the maximum blue color of indophenol, required 5 min at 60 °C, and absorbance was measured at 570 nm. We demonstrated that this convenient colorimetric microplate-based rapid assay can be applied to determine urea as an evaluation of renal function in biological fluids, such as urine, serum or plasma, and spent dialysate, during hemodialysis. This rapid and reliable assay system is very useful as the primary screening of urinalysis, and also useful to understand in renal function at practical training for students of clinical engineering. | |
| Keyword | (in Japanese) | (See Japanese page) |
| (in English) | microplate-based assay / urea / urease-indophenol colorimetric method / learning for renal function / / / / | |
| Reference Info. | IEICE Tech. Rep., vol. 114, no. 325, MBE2014-69, pp. 55-58, Nov. 2014. | |
| Paper # | MBE2014-69 | |
| Date of Issue | 2014-11-14 (MBE) | |
| ISSN | Print edition: ISSN 0913-5685 Online edition: ISSN 2432-6380 | |
| Copyright and reproduction |
All rights are reserved and no part of this publication may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopy, recording, or any information storage and retrieval system, without permission in writing from the publisher. Notwithstanding, instructors are permitted to photocopy isolated articles for noncommercial classroom use without fee. (License No.: 10GA0019/12GB0052/13GB0056/17GB0034/18GB0034) | |
| Download PDF | MBE2014-69 |
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| Conference Information | |
| Committee | MBE NC |
| Conference Date | 2014-11-21 - 2014-11-22 |
| Place (in Japanese) | (See Japanese page) |
| Place (in English) | Tohoku University |
| Topics (in Japanese) | (See Japanese page) |
| Topics (in English) | |
| Paper Information | |
| Registration To | MBE |
| Conference Code | 2014-11-MBE-NC |
| Language | English |
| Title (in Japanese) | (See Japanese page) |
| Sub Title (in Japanese) | (See Japanese page) |
| Title (in English) | Simple and Rapid Renal Function Analysis Using Newly Conducted Urea Microassay |
| Sub Title (in English) | |
| Keyword(1) | microplate-based assay |
| Keyword(2) | urea |
| Keyword(3) | urease-indophenol colorimetric method |
| Keyword(4) | learning for renal function |
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| 1st Author's Name | Toru Yokoyama |
| 1st Author's Affiliation | Sapporo College of Medical, Welfare and Dental Technology (Sapporo College of Medical, Welfare and Dental Tech.) |
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| Speaker | Author-1 |
| Date Time | 2014-11-22 09:50:00 |
| Presentation Time | 25 minutes |
| Registration for | MBE |
| Paper # | MBE2014-69 |
| Volume (vol) | vol.114 |
| Number (no) | no.325 |
| Page | pp.55-58 |
| #Pages | 4 |
| Date of Issue | 2014-11-14 (MBE) |
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