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Paper Abstract and Keywords
Presentation 2014-01-25 11:45
Multiplex detection of DNA and RNA viruses using RT-LAMP and metal coated hollow fiber membrane
Ryo Oi, Toshitsugu Sugawara, Satoshi Kuroda, Junji Arisawa, Kazuyuki Kimura (Hokkaido Inst. of Tech.) MBE2013-110
Abstract (in Japanese) (See Japanese page) 
(in English) We proposed a gene amplification method that enables the detection against small amount of viruses. It is intended for application to nucleic acid amplification test (NAT) that is performed on a subject plasma samples for the Japanese Red Cross Society to ensure the safety of the blood supply. A metal coated hollow fiber membrane was used for detection of virus. The membrane was able to isolate nucleic acids after concentration of virus. Isolated nucleic acids were applied to LAMP(Loop-Mediated Isothermal Amplification). This method was rapid and sensitive detection. In this report, to detect DNA and RNA viruses in one step, we tried to apply nucleic acids of DNA or RNA virus in one step. RT-LAMP could also detect DNA or RNA virus in one step. As a result, be detectable both DNA and RNA viruses also stable were found.
Keyword (in Japanese) (See Japanese page) 
(in English) blood supply / metal-coated hollow fiber membrane / RT-LAMP / Multiplex detection / / / /  
Reference Info. IEICE Tech. Rep., vol. 113, no. 409, MBE2013-110, pp. 79-83, Jan. 2014.
Paper # MBE2013-110 
Date of Issue 2014-01-17 (MBE) 
ISSN Print edition: ISSN 0913-5685  Online edition: ISSN 2432-6380
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All rights are reserved and no part of this publication may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopy, recording, or any information storage and retrieval system, without permission in writing from the publisher. Notwithstanding, instructors are permitted to photocopy isolated articles for noncommercial classroom use without fee. (License No.: 10GA0019/12GB0052/13GB0056/17GB0034/18GB0034)
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Conference Information
Committee MBE  
Conference Date 2014-01-24 - 2014-01-25 
Place (in Japanese) (See Japanese page) 
Place (in English) Saga University 
Topics (in Japanese) (See Japanese page) 
Topics (in English)  
Paper Information
Registration To MBE 
Conference Code 2014-01-MBE 
Language Japanese 
Title (in Japanese) (See Japanese page) 
Sub Title (in Japanese) (See Japanese page) 
Title (in English) Multiplex detection of DNA and RNA viruses using RT-LAMP and metal coated hollow fiber membrane 
Sub Title (in English)  
Keyword(1) blood supply  
Keyword(2) metal-coated hollow fiber membrane  
Keyword(3) RT-LAMP  
Keyword(4) Multiplex detection  
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1st Author's Name Ryo Oi  
1st Author's Affiliation Hokkaido Institute of technology (Hokkaido Inst. of Tech.)
2nd Author's Name Toshitsugu Sugawara  
2nd Author's Affiliation Hokkaido Institute of technology (Hokkaido Inst. of Tech.)
3rd Author's Name Satoshi Kuroda  
3rd Author's Affiliation Hokkaido Institute of technology (Hokkaido Inst. of Tech.)
4th Author's Name Junji Arisawa  
4th Author's Affiliation Hokkaido Institute of technology (Hokkaido Inst. of Tech.)
5th Author's Name Kazuyuki Kimura  
5th Author's Affiliation Hokkaido Institute of technology (Hokkaido Inst. of Tech.)
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Speaker
Date Time 2014-01-25 11:45:00 
Presentation Time 25 
Registration for MBE 
Paper # IEICE-MBE2013-110 
Volume (vol) IEICE-113 
Number (no) no.409 
Page pp.79-83 
#Pages IEICE-5 
Date of Issue IEICE-MBE-2014-01-17 


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