Paper Abstract and Keywords |
Presentation |
2011-01-28 11:25
RNA virus ditection used Metal coated hollow fiber membrane and Reverse Transcription Loop-mediated Isothermal Amplification from simulated serum sample Shinya Hayashi, Toshitsugu Sugawara, Junji Arisawa, Kazuyuki Kimura (HIT), Osamu Igarashi (NIX) MBE2010-93 |
Abstract |
(in Japanese) |
(See Japanese page) |
(in English) |
Japanese Red Cross Society has carried out Nucleic acid Amplification Test (NAT) to secure safety of a blood supply. However, NAT can not detect virus under the low degree contamination of the blood. Therefore, we started development of sensitive virus detection with uniquely contrived metal-coated hollow fiber membrane and RT-LAMP. We examined whether this virus detection could detect feline calicivirus (FCV) of RNA virus such as hepatitis C virus (HCV) or human immunodeficiency virus (HIV). As a next step, we tried to detect RNA virus from simulated serum sample. As a result, we could detect from simulated serum sample containing 5 PFU/mL of FCV and more quickly than NAT. |
Keyword |
(in Japanese) |
(See Japanese page) |
(in English) |
blood supply / metal-coated hollow fiber membrane / RT-LAMP / RNA virus / simulated serum sample / / / |
Reference Info. |
IEICE Tech. Rep., vol. 110, no. 399, MBE2010-93, pp. 77-82, Jan. 2011. |
Paper # |
MBE2010-93 |
Date of Issue |
2011-01-20 (MBE) |
ISSN |
Print edition: ISSN 0913-5685 Online edition: ISSN 2432-6380 |
Copyright and reproduction |
All rights are reserved and no part of this publication may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopy, recording, or any information storage and retrieval system, without permission in writing from the publisher. Notwithstanding, instructors are permitted to photocopy isolated articles for noncommercial classroom use without fee. (License No.: 10GA0019/12GB0052/13GB0056/17GB0034/18GB0034) |
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MBE2010-93 |
Conference Information |
Committee |
MBE |
Conference Date |
2011-01-27 - 2011-01-28 |
Place (in Japanese) |
(See Japanese page) |
Place (in English) |
Kagoshima University |
Topics (in Japanese) |
(See Japanese page) |
Topics (in English) |
ME in general |
Paper Information |
Registration To |
MBE |
Conference Code |
2011-01-MBE |
Language |
Japanese |
Title (in Japanese) |
(See Japanese page) |
Sub Title (in Japanese) |
(See Japanese page) |
Title (in English) |
RNA virus ditection used Metal coated hollow fiber membrane and Reverse Transcription Loop-mediated Isothermal Amplification from simulated serum sample |
Sub Title (in English) |
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Keyword(1) |
blood supply |
Keyword(2) |
metal-coated hollow fiber membrane |
Keyword(3) |
RT-LAMP |
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RNA virus |
Keyword(5) |
simulated serum sample |
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1st Author's Name |
Shinya Hayashi |
1st Author's Affiliation |
Graduate School of Hokkaido Institute of Technology (HIT) |
2nd Author's Name |
Toshitsugu Sugawara |
2nd Author's Affiliation |
Hokkaido Institute of Technology (HIT) |
3rd Author's Name |
Junji Arisawa |
3rd Author's Affiliation |
Hokkaido Institute of Technology (HIT) |
4th Author's Name |
Kazuyuki Kimura |
4th Author's Affiliation |
Hokkaido Institute of Technology (HIT) |
5th Author's Name |
Osamu Igarashi |
5th Author's Affiliation |
NIX Inc. (NIX) |
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Speaker |
Author-1 |
Date Time |
2011-01-28 11:25:00 |
Presentation Time |
25 minutes |
Registration for |
MBE |
Paper # |
MBE2010-93 |
Volume (vol) |
vol.110 |
Number (no) |
no.399 |
Page |
pp.77-82 |
#Pages |
6 |
Date of Issue |
2011-01-20 (MBE) |
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