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| Presentation | 2010-03-11 11:05 Changes in the concentrations of Ca2+ and Mg2+ induced by high-frequency electrical stimulation in adult rat cardiomyocytes. Hiroyuki Igarashi, Yusuke Mizutani, Sadahiro Iwabuchi, Daisuke Matsuyama, Koichi Kawahara (Hokkaido Univ.)  MBE2009-123 |
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| Abstract | (in Japanese) | (See Japanese page) |
| (in English) | Ventricular fibrillation (VF) and reentrant ventricular tachycardia (VT) are the major immediate causes of sudden cardiac death. Whereas VT is considered a rapid but well organized process, VF has been described as turbulent cardiac electrical activity, resulting from the random and aperiodic propagation of multiple independent wavelets throughout the cardiac muscle. Clinical studies have shown that VF is almost always preceded by VT of variable duration, from a few to many beats. However, the manner in which VT converts to VF, or vice versa is not yet fully understood despite more than a century of intensive study. We have previously demonstrated that the perfusion of isolated hearts with ruthenium red (RR), a blocker of the mitochondrial Ca2+ uniporter, converts pacing-induced sustained VF to VT. In addition, we have also demonstrated that the perfusion of isolated rat hearts with RR during sustained VF results in the close balance between myocardial energy supply and demand, suggesting that the balance was lost during VF, leading to the significant decrease of cellular ATP in the myocardium during VF. In this study, we investigated whether high-frequency electrical stimulation affected the intracellular concentration of Ca2+ and Mg2+ dynamics in isolated adult rat cardiomyocytes. The present study revealed that the persistent high-frequency stimulation resulted in the collapse in an ionic homeostasis in cardiomyocytes. In addition, the intracellular concentration of Mg2+ increased significantly in association with the high-frequency stimulation –induced hypercontraction, suggesting that the myocardial ATP content was markedly reduced during the stimulation. | |
| Keyword | (in Japanese) | (See Japanese page) |
| (in English) | cardiomyocytes / electrical stimulation / contraction / [Ca2+]i / [Mg2+]i / / / | |
| Reference Info. | IEICE Tech. Rep., vol. 109, no. 460, MBE2009-123, pp. 123-125, March 2010. | |
| Paper # | MBE2009-123 | |
| Date of Issue | 2010-03-02 (MBE) | |
| ISSN | Print edition: ISSN 0913-5685 Online edition: ISSN 2432-6380 | |
| Copyright and reproduction |
All rights are reserved and no part of this publication may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopy, recording, or any information storage and retrieval system, without permission in writing from the publisher. Notwithstanding, instructors are permitted to photocopy isolated articles for noncommercial classroom use without fee. (License No.: 10GA0019/12GB0052/13GB0056/17GB0034/18GB0034) | |
| Download PDF | MBE2009-123 |
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| Conference Information | |
| Committee | NC MBE |
| Conference Date | 2010-03-09 - 2010-03-11 |
| Place (in Japanese) | (See Japanese page) |
| Place (in English) | Tamagawa University |
| Topics (in Japanese) | (See Japanese page) |
| Topics (in English) | General |
| Paper Information | |
| Registration To | MBE |
| Conference Code | 2010-03-NC-MBE |
| Language | Japanese |
| Title (in Japanese) | (See Japanese page) |
| Sub Title (in Japanese) | (See Japanese page) |
| Title (in English) | Changes in the concentrations of Ca2+ and Mg2+ induced by high-frequency electrical stimulation in adult rat cardiomyocytes. |
| Sub Title (in English) | |
| Keyword(1) | cardiomyocytes |
| Keyword(2) | electrical stimulation |
| Keyword(3) | contraction |
| Keyword(4) | [Ca2+]i |
| Keyword(5) | [Mg2+]i |
| Keyword(6) | |
| Keyword(7) | |
| Keyword(8) | |
| 1st Author's Name | Hiroyuki Igarashi |
| 1st Author's Affiliation | Hokkaido University (Hokkaido Univ.) |
| 2nd Author's Name | Yusuke Mizutani |
| 2nd Author's Affiliation | Hokkaido University (Hokkaido Univ.) |
| 3rd Author's Name | Sadahiro Iwabuchi |
| 3rd Author's Affiliation | Hokkaido University (Hokkaido Univ.) |
| 4th Author's Name | Daisuke Matsuyama |
| 4th Author's Affiliation | Hokkaido University (Hokkaido Univ.) |
| 5th Author's Name | Koichi Kawahara |
| 5th Author's Affiliation | Hokkaido University (Hokkaido Univ.) |
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| Speaker | Author-1 |
| Date Time | 2010-03-11 11:05:00 |
| Presentation Time | 25 minutes |
| Registration for | MBE |
| Paper # | MBE2009-123 |
| Volume (vol) | vol.109 |
| Number (no) | no.460 |
| Page | pp.123-125 |
| #Pages | 3 |
| Date of Issue | 2010-03-02 (MBE) |
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