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| Presentation | 2007-09-21 16:15 Selection of hapten structures for highly sensitive detection of TNT using SPR immunosensor by indirect competitive method Yutaka Mizuta, Takeshi Onodera, Praveen Singh, Norio Miura, Kiyoshi Matsumoto, Kiyoshi Toko (Kyushu Univ.)  ED2007-155 OME2007-42 Link to ES Tech. Rep. Archives: ED2007-155 OME2007-42 |
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| Abstract | (in Japanese) | (See Japanese page) |
| (in English) | In order to detect trinitrotoluene (TNT) with high sensitivity, SPR immunosensor with indirect competitive method was used. In the development of the sensor chip, dithiol self-assembled monolayers were formed on the Au surface, and TNT analogues were attached to them through polyethylene glycol chain to reduce non specific adsorption of protein. Three kinds of TNT analogues were immobilized to the sensor chip surfaces and we examined whether there is a significant influence of the hapten structure and their affinity to TNT antibody on the sensitivity of TNT detection. | |
| Keyword | (in Japanese) | (See Japanese page) |
| (in English) | SPR / Indirect competitive assay / TNT / Nonspecific adsorption / Hapten / Affinity constant / / | |
| Reference Info. | IEICE Tech. Rep., vol. 107, no. 227, ED2007-155, pp. 59-64, Sept. 2007. | |
| Paper # | ED2007-155 | |
| Date of Issue | 2007-09-14 (ED, OME) | |
| ISSN | Print edition: ISSN 0913-5685 Online edition: ISSN 2432-6380 | |
| Copyright and reproduction |
All rights are reserved and no part of this publication may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopy, recording, or any information storage and retrieval system, without permission in writing from the publisher. Notwithstanding, instructors are permitted to photocopy isolated articles for noncommercial classroom use without fee. (License No.: 10GA0019/12GB0052/13GB0056/17GB0034/18GB0034) | |
| Download PDF | ED2007-155 OME2007-42 Link to ES Tech. Rep. Archives: ED2007-155 OME2007-42 |
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| Conference Information | |
| Committee | ED OME |
| Conference Date | 2007-09-21 - 2007-09-21 |
| Place (in Japanese) | (See Japanese page) |
| Place (in English) | Kyushu Institute of Technology |
| Topics (in Japanese) | (See Japanese page) |
| Topics (in English) | |
| Paper Information | |
| Registration To | ED |
| Conference Code | 2007-09-ED-OME |
| Language | Japanese |
| Title (in Japanese) | (See Japanese page) |
| Sub Title (in Japanese) | (See Japanese page) |
| Title (in English) | Selection of hapten structures for highly sensitive detection of TNT using SPR immunosensor by indirect competitive method |
| Sub Title (in English) | |
| Keyword(1) | SPR |
| Keyword(2) | Indirect competitive assay |
| Keyword(3) | TNT |
| Keyword(4) | Nonspecific adsorption |
| Keyword(5) | Hapten |
| Keyword(6) | Affinity constant |
| Keyword(7) | |
| Keyword(8) | |
| 1st Author's Name | Yutaka Mizuta |
| 1st Author's Affiliation | Kyushu University (Kyushu Univ.) |
| 2nd Author's Name | Takeshi Onodera |
| 2nd Author's Affiliation | Kyushu University (Kyushu Univ.) |
| 3rd Author's Name | Praveen Singh |
| 3rd Author's Affiliation | Kyushu University (Kyushu Univ.) |
| 4th Author's Name | Norio Miura |
| 4th Author's Affiliation | Kyushu University (Kyushu Univ.) |
| 5th Author's Name | Kiyoshi Matsumoto |
| 5th Author's Affiliation | Kyushu University (Kyushu Univ.) |
| 6th Author's Name | Kiyoshi Toko |
| 6th Author's Affiliation | Kyushu University (Kyushu Univ.) |
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| Speaker | Author-1 |
| Date Time | 2007-09-21 16:15:00 |
| Presentation Time | 25 minutes |
| Registration for | ED |
| Paper # | ED2007-155, OME2007-42 |
| Volume (vol) | vol.107 |
| Number (no) | no.227(ED), no.228(OME) |
| Page | pp.59-64 |
| #Pages | 6 |
| Date of Issue | 2007-09-14 (ED, OME) |
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