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Paper Abstract and Keywords
Presentation 2006-01-27 10:25
Application of the synaptic bouton preparation to rat retinal ganglion cells
Tamami Motomura, Yuki Hayashida (Kumamoto Univ.), Norio Akaike (Kumamoto Health Science Univ.), Nobuki Murayama (Kumamoto Univ.)
Abstract (in Japanese) (See Japanese page) 
(in English) The vibrodissociation method has been applied to various CNS regions, yet never applied to vertebrate retinas. This study is aimed to examine whether this method is valid to isolate ganglion cells from a mammalian retina. In perforated-patch whole-cell recordings, the isolated ganglion cells showed sustained as well as transient spiking in response to depolarizing constant current injections, and showed voltage-gated Na+ current in response to depolarization steps. Under current-clamp, the ganglion cells somata showed spontaneous IPSP-like events of which reversal potential was approximately -80 mV. The present study suggested that the vibrodissociation method is useful to isolate rat retinal ganglion cells attached with inhibitory synaptic boutons.
Keyword (in Japanese) (See Japanese page) 
(in English) retina / ganglion cell / synaptic bouton preparation / electrophysiological experiment / / / /  
Reference Info. IEICE Tech. Rep., vol. 105, no. 577, MBE2005-108, pp. 5-8, Jan. 2006.
Paper # MBE2005-108 
Date of Issue 2006-01-20 (MBE) 
ISSN Print edition: ISSN 0913-5685  Online edition: ISSN 2432-6380
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Conference Information
Committee MBE  
Conference Date 2006-01-27 - 2006-01-28 
Place (in Japanese) (See Japanese page) 
Place (in English)  
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Paper Information
Registration To MBE 
Conference Code 2006-01-MBE 
Language Japanese 
Title (in Japanese) (See Japanese page) 
Sub Title (in Japanese) (See Japanese page) 
Title (in English) Application of the synaptic bouton preparation to rat retinal ganglion cells 
Sub Title (in English)  
Keyword(1) retina  
Keyword(2) ganglion cell  
Keyword(3) synaptic bouton preparation  
Keyword(4) electrophysiological experiment  
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1st Author's Name Tamami Motomura  
1st Author's Affiliation Kumamoto University (Kumamoto Univ.)
2nd Author's Name Yuki Hayashida  
2nd Author's Affiliation Kumamoto University (Kumamoto Univ.)
3rd Author's Name Norio Akaike  
3rd Author's Affiliation Kumamoto Health Science University (Kumamoto Health Science Univ.)
4th Author's Name Nobuki Murayama  
4th Author's Affiliation Kumamoto University (Kumamoto Univ.)
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Speaker
Date Time 2006-01-27 10:25:00 
Presentation Time 25 
Registration for MBE 
Paper # IEICE-MBE2005-108 
Volume (vol) IEICE-105 
Number (no) no.577 
Page pp.5-8 
#Pages IEICE-4 
Date of Issue IEICE-MBE-2006-01-20 


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