| Paper Abstract and Keywords |
| Presentation |
2004-06-18 14:25
The characteristics of endothelial cell-smooth muscle cell cocultures in LDL-uptake and cell proliferation
Naoya Nakada, Mayuko Minamiyama, Koichi Niwa, Takeshi Karino (Hokkaido Univ.) |
| Abstract |
(in Japanese) |
(See Japanese page) |
| (in English) |
An attempt was made to construct an EC-SMC coculture as a model of an arterial wall by seeding bovine arterial endothelial cells (ECs) directly over smooth muscle cells (SMCs) and coculturing them. It was found that to construct an EC-SMC coculture in which ECs are in direct contact with SMCs and covering them as a confluent monolayer, it is necessary to have ascorbic acid at a concentration higher than 50 mg/mL. It was also found that both the uptake of LDL by the cells in the EC-SMC coculture and the thickness of the cell layer of the EC-SMC coculture increased with increasing the concentration of LDL in a culture medium. |
| Keyword |
(in Japanese) |
(See Japanese page) |
| (in English) |
Endothelial cell / Smooth muscle cell / Coculture / LDL / Ascorbic acid / Collagen / / |
| Reference Info. |
IEICE Tech. Rep., vol. 104, no. 129, MBE2004-14, pp. 13-15, June 2004. |
| Paper # |
MBE2004-14 |
| Date of Issue |
2004-06-11 (MBE) |
| ISSN |
Print edition: ISSN 0913-5685 |
| Download PDF |
|
| Conference Information |
| Committee |
MBE |
| Conference Date |
2004-06-18 - 2004-06-18 |
| Place (in Japanese) |
(See Japanese page) |
| Place (in English) |
Hokkaido University |
| Topics (in Japanese) |
(See Japanese page) |
| Topics (in English) |
|
| Paper Information |
| Registration To |
MBE |
| Conference Code |
2004-06-MBE |
| Language |
Japanese |
| Title (in Japanese) |
(See Japanese page) |
| Sub Title (in Japanese) |
(See Japanese page) |
| Title (in English) |
The characteristics of endothelial cell-smooth muscle cell cocultures in LDL-uptake and cell proliferation |
| Sub Title (in English) |
|
| Keyword(1) |
Endothelial cell |
| Keyword(2) |
Smooth muscle cell |
| Keyword(3) |
Coculture |
| Keyword(4) |
LDL |
| Keyword(5) |
Ascorbic acid |
| Keyword(6) |
Collagen |
| Keyword(7) |
|
| Keyword(8) |
|
| 1st Author's Name |
Naoya Nakada |
| 1st Author's Affiliation |
Hokkaido University (Hokkaido Univ.) |
| 2nd Author's Name |
Mayuko Minamiyama |
| 2nd Author's Affiliation |
Hokkaido University (Hokkaido Univ.) |
| 3rd Author's Name |
Koichi Niwa |
| 3rd Author's Affiliation |
Hokkaido University (Hokkaido Univ.) |
| 4th Author's Name |
Takeshi Karino |
| 4th Author's Affiliation |
Hokkaido University (Hokkaido Univ.) |
| 5th Author's Name |
|
| 5th Author's Affiliation |
() |
| 6th Author's Name |
|
| 6th Author's Affiliation |
() |
| 7th Author's Name |
|
| 7th Author's Affiliation |
() |
| 8th Author's Name |
|
| 8th Author's Affiliation |
() |
| 9th Author's Name |
|
| 9th Author's Affiliation |
() |
| 10th Author's Name |
|
| 10th Author's Affiliation |
() |
| 11th Author's Name |
|
| 11th Author's Affiliation |
() |
| 12th Author's Name |
|
| 12th Author's Affiliation |
() |
| 13th Author's Name |
|
| 13th Author's Affiliation |
() |
| 14th Author's Name |
|
| 14th Author's Affiliation |
() |
| 15th Author's Name |
|
| 15th Author's Affiliation |
() |
| 16th Author's Name |
|
| 16th Author's Affiliation |
() |
| 17th Author's Name |
|
| 17th Author's Affiliation |
() |
| 18th Author's Name |
|
| 18th Author's Affiliation |
() |
| 19th Author's Name |
|
| 19th Author's Affiliation |
() |
| 20th Author's Name |
|
| 20th Author's Affiliation |
() |
| Speaker |
Author-1 |
| Date Time |
2004-06-18 14:25:00 |
| Presentation Time |
25 minutes |
| Registration for |
MBE |
| Paper # |
MBE2004-14 |
| Volume (vol) |
vol.104 |
| Number (no) |
no.129 |
| Page |
pp.13-15 |
| #Pages |
3 |
| Date of Issue |
2004-06-11 (MBE) |