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| Presentation | 2008-09-26 10:00 Image analysis of higher-order nuclear architecture formed by a DNA recombinational repair protein RAD51 Akira Asano, Kazuteru Kono, Satoshi Tashiro (Hiroshima Univ.)  SIP2008-97 SIS2008-32 |
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| Abstract | (in Japanese) | (See Japanese page) |
| (in English) | It has been recently shown that the cell nucleus is a highly compartmentalized organelle with chromosome and chromatin domains formed by DNA. Although nuclear proteins also have been speculated to form higher order structure in the compartment between chromatin domains, how the formation of interchromatin compartment is organized is still unclear. We have previously shown that RAD51, a recombinational repair protein, forms nuclear foci, typically during S phase, and accumulates at sites of DNA double-strand breaks. We found that over-expressed RAD51 forms bundle-like structures in the nucleus. Since the structures are formed in the interchromatin compartment, we decided to study the influence of chromatin structure to the organization of interchromatin compartment. For this purpose, we examined the change of the RAD51 bundle-like structure shape after de-condensation of chromatin by the treatment with trichostatin A (TSA), a histone deacetylase inhibitor. The complexity of the GFP-RAD51 bundle-like structures was analyzed quantitatively using digital image processing methods. | |
| Keyword | (in Japanese) | (See Japanese page) |
| (in English) | recombinatorial repair protein / chromatin structure / bioimaging / / / / / | |
| Reference Info. | IEICE Tech. Rep., vol. 108, no. 213, SIP2008-97, pp. 43-46, Sept. 2008. | |
| Paper # | SIP2008-97 | |
| Date of Issue | 2008-09-18 (SIP, SIS) | |
| ISSN | Print edition: ISSN 0913-5685 Online edition: ISSN 2432-6380 | |
| Copyright and reproduction |
All rights are reserved and no part of this publication may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopy, recording, or any information storage and retrieval system, without permission in writing from the publisher. Notwithstanding, instructors are permitted to photocopy isolated articles for noncommercial classroom use without fee. (License No.: 10GA0019/12GB0052/13GB0056/17GB0034/18GB0034) | |
| Download PDF | SIP2008-97 SIS2008-32 |
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| Conference Information | |
| Committee | SIS SIP IPSJ-AVM |
| Conference Date | 2008-09-25 - 2008-09-26 |
| Place (in Japanese) | (See Japanese page) |
| Place (in English) | Tobata Campus, Kyushu Institute of Technology |
| Topics (in Japanese) | (See Japanese page) |
| Topics (in English) | Signal Processing, Intelligent Multimedia Systems, etc. |
| Paper Information | |
| Registration To | SIP |
| Conference Code | 2008-09-SIS-SIP-AVM |
| Language | Japanese |
| Title (in Japanese) | (See Japanese page) |
| Sub Title (in Japanese) | (See Japanese page) |
| Title (in English) | Image analysis of higher-order nuclear architecture formed by a DNA recombinational repair protein RAD51 |
| Sub Title (in English) | |
| Keyword(1) | recombinatorial repair protein |
| Keyword(2) | chromatin structure |
| Keyword(3) | bioimaging |
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| 1st Author's Name | Akira Asano |
| 1st Author's Affiliation | Hiroshima University (Hiroshima Univ.) |
| 2nd Author's Name | Kazuteru Kono |
| 2nd Author's Affiliation | Hiroshima University (Hiroshima Univ.) |
| 3rd Author's Name | Satoshi Tashiro |
| 3rd Author's Affiliation | Hiroshima University (Hiroshima Univ.) |
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| Speaker | Author-1 |
| Date Time | 2008-09-26 10:00:00 |
| Presentation Time | 25 minutes |
| Registration for | SIP |
| Paper # | SIP2008-97, SIS2008-32 |
| Volume (vol) | vol.108 |
| Number (no) | no.213(SIP), no.214(SIS) |
| Page | pp.43-46 |
| #Pages | 4 |
| Date of Issue | 2008-09-18 (SIP, SIS) |
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